In a study of the effect of the widely used, commercially available Enterococcus faecium BIO probiotic on enteropathogen growth, we determined the effect using a new, growth inhibition test on layered agar medium. E. faecium BIO and test organisms did not come into direct mutual contact and effects were judged by test organism growth on the agar surface. E. faecium BIO showed antibiotic effects on all pathogens tested. We also confirmed that E. faecium BIO suppressed organism growth mainly by producing lactic acid, but due to lactate alone.
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