AI Article Synopsis
- The WAVE regulatory complex (WRC) helps transmit signals from Rac GTPase to the Arp2/3 complex, which is involved in actin polymerization.
- We successfully reconstituted WRC from humans and Drosophila, finding it inactive until activated by Rac, which depends on nucleotide presence.
- Our findings clarify how WAVE is inhibited, resolve existing conflicting theories, and highlight shared regulatory mechanisms among proteins in the WAVE/WASP family.
Article Abstract
The WAVE regulatory complex (WRC) transmits information from the Rac GTPase to the actin nucleator Arp2/3 complex. We have reconstituted recombinant human and Drosophila WRC in several forms and shown that they are inactive toward Arp2/3 complex but can be activated by Rac in a nucleotide-dependent fashion. Our observations identify core components needed for WAVE inhibition, reconcile contradictory existing mechanisms and reveal common regulatory principles for the WAVE/WASP family of proteins.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2716658 | PMC |
| http://dx.doi.org/10.1038/nsmb.1587 | DOI Listing |
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