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Trehalose Cryopreservation of Human Mesenchymal Stem Cells from Cord Tissue.
Biopreserv Biobank
December 2024
Coordinating Node, Andalusian Public Health System Biobank, Granada, Spain.
Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is of fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application of hMSCs necessitates hypothermic storage for long periods, a process that requires the use of non-toxic and efficient cryo-reagents capable of maintaining high viability and differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide (DMSO), which have been shown to be toxic for clinical applications.
View Article and Find Full Text PDFCarboxymethyl chitosan: Synthesis, functional properties, and applications in sustainable food packaging material.
Compr Rev Food Sci Food Saf
November 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China.
Global environmental concerns about non-degradable packaging materials are increasing. Carboxymethyl chitosan (CMCS), a polysaccharide widely used in the food industry, has gained attention in the field of food packaging. Due to its biodegradability, film-forming ability, and biocompatibility, CMCS has emerged as a sustainable option for degradable and functional food packaging materials, offering solutions to plastic pollution and food waste issues.
View Article and Find Full Text PDFSoft-lithographically defined template for arbitrarily patterned acoustic bioassembly.
Biofabrication
December 2024
Tissue Engineering and Organ Manufacturing (TEOM) Lab, Department of Biomedical Engineering, TaiKang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan, Hubei 430071, People's Republic of China.
Acoustic bioassembly is recently regarded as a highly efficient biofabrication tool to generate functional tissue mimics. Despite their capacity of directly patterning live cells with close intercellular proximity, most acoustic bioassembly techniques are currently limited to generate some specific simple types of periodic and symmetric patterns, which represents an urgent challenge to emulate geometrically complex cytoarchitecture in human tissue. To address this challenge, we herein demonstrate a soft-lithographically defined acoustic bioassembly (SLAB) technique that enables to assemble live cells into geometrically defined arbitrary multicellular structures.
View Article and Find Full Text PDFTailoring cell therapies for diabetic metabolic phenotypes: a comparative study on the efficacy of various umbilical cord-derived cell regimens.
Stem Cells Transl Med
November 2024
Department of Endocrinology and Metabolism, Qilu Hospital, Shandong University, Jinan 250012, Shandong, People's Republic of China.
Given the high heterogeneity of type 2 diabetes mellitus (T2DM), it is imperative to develop personalized stem cell infusion regimen for targeted metabolic phenotype in order to ensure optimal therapeutic efficacy. In this study, we conducted a comparative analysis of 4 infusion regimens involving single and repeated infusions of human umbilical cord Wharton's jelly-derived MSCs (hucMSCs), single infusions of umbilical cord blood mononuclear cells (UCB), and sequential infusions of hucMSCs and UCB in T2DM rats. Results showed all 4 infusion regimens exhibited comparable efficacy in lowering fasting blood glucose levels and suppressing glucagon secretion.
View Article and Find Full Text PDFKnowledge, Attitudes, and Practices of Pregnant Women and Hospital Staff Regarding Umbilical Cord Blood Banking: Systematic Review and Meta-Analysis.
Healthcare (Basel)
October 2024
Department of Woman, Child and General and Specialized Surgery, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Article Synopsis
- - This study aimed to evaluate how much pregnant women and hospital staff know about umbilical cord blood (UCB) donation and storage, revealing key insights into their views and practices.
- - A total of 19 studies involving nearly 20,000 pregnant women and over 1,200 hospital staff showed that awareness of UCB was 61% for both groups, with 57% of pregnant women having a positive attitude toward UCB donation.
- - The findings indicated a strong preference for public UCB banking over private options, with 51% of pregnant women favoring public storage compared to only 12% for private storage.
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