Some Bacillus thuringiensis strains secrete beta-exotoxin, which is an insecticidal, thermostable adenine nucleotide analogue. Discrepancies between detection of beta-exotoxin by high-performance liquid chromatography and insect bioassays have shown the importance of bioassays in the determination of beta-exotoxin production. With the aim of improving the fly beta-exotoxin bioassay, a range of fly diets were evaluated and the best performing diet was incorporated into a novel beta-exotoxin bioassay. The improved bioassay is characterised by good control pupation percentages, low variability, easy setup and monitoring. The bioassay allowed unambiguous differentiation between beta-exotoxin producing and non-producing strains, and is suitable for the routine screening of B. thuringiensis strains for beta-exotoxins.
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| http://dx.doi.org/10.1016/j.jip.2009.03.007 | DOI Listing |
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