A review. Transfection is a method of cell transformation based on the introduction of a plasmid encoding a protein or RNA into the living cells. Basic methods of transfection and their strengths and weaknesses are discussed. A large part of the review is focused on the lentiviral transduction as one of the most efficient methods of transformation of nerve cells. The development of modern systems of lentiviral transfection is traced and a number of works performed with the use of in vivo and in vitro lentiviral transfection of the nervous system are reviewed.
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Lanthanide-tetrazine probes for bio-imaging and click chemistry.
Chem Sci
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Department of Chemistry, Imperial College London Molecular Sciences Research Hub, 82 Wood Lane, White City Campus London W12 0BZ UK
The blood-brain-barrier prevents many imaging agents and therapeutics from being delivered to the brain that could fight central nervous system diseases such as Alzheimer's disease and strokes. However, techniques such as the use of stapled peptides or peptide shuttles may allow payloads through, with bioconjugation achieved bio-orthogonal tetrazine/norbornene click chemistry. A series of lanthanide-tetrazine probes have been synthesised herein which could be utilised in bio-orthogonal click chemistry with peptide-based delivery systems to deliver MRI agents through the blood-brain-barrier.
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View Article and Find Full Text PDFMechanisms of Cancer-Induced Bone Pain.
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Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.
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Front Neurosci
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