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Cell and determinant markers are important in fish immunology and have vast applications in aquaculture but the availability of such markers are quite limited. Hence, there is a need to identify and also further improve existing markers in fish. Here, we developed effective polyclonal antibodies (pAbs) targeting specific parts of the Japanese flounder (Paralichthys olivaceus) IgM constant (C) region. Recombinant proteins from the CHmu2 and CHmu3 termed IgM fragment 1 (rIgM1) and from CHmu4 termed IgM fragment 2 (rIgM2) were expressed and used to construct mouse pAb-IgM1 and pAb-IgM2, respectively. pAb-IgM1 detected both the approximately 77 kDa and the approximately 72 kDa heavy chains detected while pAb-IgM2 marked only the approximately 77 kDa heavy chain of Japanese flounder. Both pAbs detected IgM heavy chain in immune-related tissues, heart and serum. pAb-IgM2, but not pAb-IgM1, revealed cross reactions with other fish species detecting pronounced multiple IgM bands suggesting that the CHmu4 is an important functional region in the teleost IgM molecules. Finally, the pAb-IgMs detected surface IgM+ (sIgM+) and cytoplasmic IgM+ (cIgM+) B cells in Japanese flounder kidney in vivo.
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http://dx.doi.org/10.1016/j.fsi.2009.03.008 | DOI Listing |
J Environ Sci (China)
June 2025
Laboratory of Pathology and Immunology of Aquatic Animals/Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China. Electronic address:
Due to the discharge of industrial wastewater, urban domestic sewage, and intensive marine aquaculture tailwater, nitrate (NO) pollution has emerged as a significant issue in offshore waters. Nitrate pollution affects aquatic life and may interact with other pollutants, leading to comprehensive toxicity. Cadmium (Cd) is the most widespread metal contaminant, adversely affecting aquatic life in the coastal waters of China.
View Article and Find Full Text PDFAnimals (Basel)
October 2024
China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Japanese flounder is usually gonochoristic, with gonads that are either testes or ovaries. Here, we report an unusual case of hermaphroditism in Japanese flounder captured from the Bohai Sea. In the intersex flounder, the membrane of the upper ovary was closely connected to the abdominal muscles and internal organs, and the eggs filled the entire abdomen.
View Article and Find Full Text PDFGen Comp Endocrinol
December 2024
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China. Electronic address:
Rbpms2, an RNA-binding protein with multiple splicing (Rbpms), can interact with RNAs to involve oocyte development, thereby influencing female sex differentiation in vertebrates. Here, two splicing variants of the Rbpms2 gene from Japanese flounder (Paralichthys olivaceus) were identified, namely Rbpms2.1 and Rbpms2.
View Article and Find Full Text PDFInt J Mol Sci
October 2024
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Ras-related associated with diabetes (RRAD) is a member of the Ras GTPase superfamily that plays a role in several cellular functions, such as cell proliferation and differentiation. In particular, the superfamily acts as an NF-κB signaling pathway inhibitor and calcium regulator to participate in the immune response pathway. A recent transcriptome study revealed that was expressed in the spleen of disease-resistant Japanese flounder () individuals compared with disease-susceptible individuals, and the results were also verified by qPCR.
View Article and Find Full Text PDFFish Shellfish Immunol
November 2024
School of Ocean, Yantai University, Yantai, China. Electronic address:
As an important CXC chemokine, CXCL8 plays pleiotropic roles in immunological response. In teleost, CXCL8 is involved in cell migration and bacterial invasion. However, the immune antibacterial function of CXCL8 in Japanese flounder (Paralichthys olivaceus) (PoCXCL8) is largely scarce.
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