A new cell analysis method, differential mobility cytometry (DMC), was developed to monitor cells spatially and temporally or to separate cells based on affinity interactions. DMC combines an oscillation system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separation volume (capillaries, channels, etc.) can be used. This unique separation approach uses oscillating flow and differential imaging to analyze cells as they retard and adhere to an affinity surface. Three main factors of the oscillation system were studied: the pump speed, oscillation frequency, and cell velocity at different oscillation speeds. The optimized oscillation frequency and intensity were determined. Cell-surface interactions were used to estimate the number of bonds formed during cell capture. An average of 200 bonds (standard deviation of 150 bonds) were formed during cell capture. The variability was due to differences in cell-capture times (0.8 +/- 0.6 s). Cells expressing the target protein on the surface oscillated slower and were captured by the corresponding ligand on the capillary surface. Cells were detected by differential imaging of a charge-coupled device camera. DMC measurements were optimized with respect to the camera frame difference. Cells were observed to slow as they reached the surface and could be observed to sway in the oscillating flow as they were tethered to the surface by a capture antibody. With the advantage of high cell-capture efficiency and temporally monitoring cell adhesion by the differential mobility of cells, DMC has proven to be a useful tool in cell analysis for basic biological studies and biomedical research.
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http://dx.doi.org/10.1021/ac900277y | DOI Listing |
Biol Pharm Bull
December 2024
Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University.
We aimed to investigate the mechanism of high mobility group box 1 (HMGB1) in the accelerated fracture healing process during Traumatic brain injury (TBI). The lateral ventricles of mice in the TBI model group were injected with adenovirus-packaged short hairpin RNA (shRNA)-HMGB1 or overexpressing (ov)-HMGB1 vector. We found HMGB1 levels were higher in bone tissue at the fracture end of TBI combined with fracture model mice.
View Article and Find Full Text PDFStem Cell Res Ther
December 2024
The Affiliated Taian City Central Hospital of Qingdao University, Taian, 271000, China.
Background: In the rat knee stiffness model, the duration of traction treatment is mostly 20-40 min; however, relatively few studies have been conducted on longer traction treatment of extended knee stiffness in rats. Therefore, the aim of this study was to explore the efficacy of prolonged traction and its mechanism of action in extended knee stiffness in rats.
Methods: The model of extended knee joint stiffness was established in rats and treated with powered flexion position traction.
J Gerontol A Biol Sci Med Sci
December 2024
Department of Health Outcomes & Biomedical Informatics, College of Medicine, University of Florida.
Background: Engaging in physical activity is critical for maintaining well-being in older adults, particularly those at heightened risk for mobility disability. We assessed the effects of a physical activity (PA) intervention on perceived stress, fatigue, and depressive symptoms compared to a health education (HE) program in older adults with mobility challenges and evaluated differential effects of the interventions among those with the poorest self-rated mental health at baseline.
Methods: Secondary data analysis of the Lifestyle Interventions and Independence for Elders (LIFE) Study, a single-blinded, parallel randomized controlled trial conducted between February 2010 and December 2013.
Front Sociol
December 2024
University of Duisburg-Essen, Duisburg, Germany.
In public debates, transnational families are portrayed as a deviation from the norm of "good childhood." In Europe, this is emphasized by the term "Euro-orphans," branding parents' (especially mothers') absence as a violation and scandalizing it. Children's voices are rarely heard in public discourse, and although research is now turning its attention to the "stayer children," they and their perspectives on transnational family life remain underrepresented, especially in Europe.
View Article and Find Full Text PDFAnal Chem
December 2024
SCIEX, Concord, Ontario L4K 4V8, Canada.
We report the first implementation of ion mobility mass spectrometry combined with an ultrahigh throughput sample introduction technology for high-throughput screening (HTS). The system integrates differential mobility spectrometry (DMS) with acoustic ejection mass spectrometry (AEMS), termed DAEMS, enabling the simultaneous quantitation of structural isomers that are the substrates and products of isomerase-mediated reactions in intermediary metabolism. We demonstrate this potential by comparing DAEMS to a luminescence assay for the isoform of phosphoglycerate mutase (iPGM) distinctively present in pathogens, offering an opportunity as a drug target for a variety of microbial and parasite borne diseases.
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