Sporothrix schenckii is known to produce DHN melanin on both conidial and yeast cells, however little information is available regarding the factors inducing fungal melanization. We evaluated whether culture conditions influenced melanization of 25 Brazilian S. schenckii strains and one control strain (ATCC 10212). Tested conditions included different media, pH, temperature, incubation time, glucose concentrations, and presence or absence of tricyclazole or L-DOPA. Melanization was reduced on Sabouraud compared to defined chemical medium. The majority of strains produced small amounts of melanin at 37 degrees C and none melanized at basic pH. Increased glucose concentrations did not inhibit melanization, rather increasing glucose enhanced pigment production in 27% of strains. Melanin synthesis was also enhanced by the addition of L-DOPA and its addition to medium with tricyclazole, an inhibitor of melanin synthesis, resulted in fungal melanization, including hyphal melanin production. Our results suggest that different S. schenckii strains have distinct control of melanization and that this fungus can use phenolic compounds to enhance melanization in vitro.
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http://dx.doi.org/10.1016/j.micinf.2009.03.002 | DOI Listing |
Case Rep Dent
January 2025
Division of Oral Medicine, Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Oral pigmentation can arise from various factors, including physiological and pathological, or as a manifestation of an underlying systemic disease. We present an atypical case of dermal filler-related complication, in which clinical lip pigmentation was observed. This condition can pose a diagnostic challenge in accurately identifying its cause.
View Article and Find Full Text PDFCureus
December 2024
Urology, Rotherham District General Hospital, Rotherham, GBR.
Bladder melanosis is a rare and poorly understood condition involving melanin pigmentation within the urothelial mucosa. Cases often present with haematuria, urinary obstructive symptoms, or cystitis. While generally considered benign, its potential association with malignancy warrants regular monitoring, as cases have previously been reported of an association with urothelial carcinoma and melanoma, although it is unclear whether there is a causal relationship.
View Article and Find Full Text PDFInsect Biochem Mol Biol
January 2025
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA. Electronic address:
Insect prophenoloxidases (proPO) are activated during immune responses by a proPO activating protease (PAP) in the presence of a high molecular weight cofactor assembled from serine protease homologs (SPH) that lack proteolytic activity. PAPs and the SPHs have a similar architecture, with an amino-terminal clip domain and a carboxyl-terminal protease domain. The SPHs belong to CLIPA subfamily of SP-related proteins.
View Article and Find Full Text PDFNew Phytol
January 2025
Amsterdam Institute for Life and Environment (A-LIFE), Section Systems Ecology, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081HV, Amsterdam, the Netherlands.
Lichens play important roles in habitat formation and community succession in polar and alpine ecosystems. Despite their significance, the ecological effects of lichen traits remain poorly researched. We propose a trait trade-off for managing light exposure based on climatic harshness.
View Article and Find Full Text PDFBr J Dermatol
January 2025
Department of Dermatology, Taiyuan Central Hospital, 030001,Taiyuan, China.
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