Simultaneous determination of four tobacco-specific N-nitrosamines (TSNA) in human urine.

J Chromatogr B Analyt Technol Biomed Life Sci

Analytisch-biologisches Forschungslabor GmbH, Goethestrasse 20, 80336 Munich, Germany.

Published: April 2009

AI Article Synopsis

  • Tobacco-specific N-nitrosamines (TSNA) such as NNK, NNN, NAB, and NAT are linked to cancer caused by tobacco smoke.
  • A new LC-MS/MS method was created to accurately measure both free and conjugated TSNA levels in human urine.
  • Smokers have notably higher TSNA levels in their urine compared to nonsmokers, demonstrating the effectiveness of this new assessment method for tobacco exposure.

Article Abstract

Tobacco-specific N-nitrosamines (TSNA) include 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT). TSNA are suggested to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC-MS/MS method for the determination of total (free and conjugated) TSNA in human urine. The limits of detection (LOD) were 2.0, 0.8, 1.1 and 0.7 pg/ml for NNAL, NNN, NAB and NAT, respectively. Smokers were found to have significantly higher levels of TSNA in their urine than nonsmokers. In conclusion, the newly developed method is suitable for assessing the tobacco use-related exposure to NNK, NNN, NAB and NAT.

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Source
http://dx.doi.org/10.1016/j.jchromb.2009.03.009DOI Listing

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