AI Article Synopsis
- Multiphoton microscopy (MPM) is a powerful technique for examining cells and their functions deep within tissues and organs.
- The introduction of infrared-MPM, using wavelengths above 1080 nm, allows for the use of red fluorophores, which improves imaging capabilities and reduces damage to the cells.
- This advanced method maintains high-resolution imaging at substantial depths, making it ideal for long-term observation of live cells in deep tissue environments.
Article Abstract
Multiphoton microscopy (MPM) is the method of choice for investigating cells and cellular functions in deep tissue sections and organs. Here we present the setup and applications of infrared-(IR-)MPM using excitation wavelengths above 1080 nm. IR-MPM enables the use of red fluorophores and fluorescent proteins, doubles imaging depth, improves second harmonic generation of tissue structures, and strongly reduces phototoxicity and photobleaching, compared with conventional MPM. Furthermore, it still provides subcellular resolution at depths of several hundred micrometers and thus will enhance long-term live cell and deep tissue microscopy.
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| http://dx.doi.org/10.1016/j.copbio.2009.02.008 | DOI Listing |
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