The prevalence of borreliae in 209 nymphal and 251 adult Ixodes ricinus was investigated in two areas of southern Moravia, Czechoslovakia, using the dark-field and the Giemsa stained-smear techniques. The proportions of infected ticks were 3.8% in nymphs and 10.6% in adults of area A, while they were 29.1% in nymphs and 35.9% in adults of area B. The mean number of borreliae per tick was about 3 to 10 times greater in adult than in nymphal I. ricinus. The results indicate a significant role of nymphal I. ricinus in the ecology of Borrelia burgdorferi and in the epidemiology and epizootiology of Lyme borreliosis.
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