Preparation of immunogens and production of antibodies.

Methods Mol Biol

Floral and Nursery Plants Research Unit, U.S. National Arboretum, USDA-ARS, Beltsville, MD 20705, USA.

Published: April 2009

The quality of reagents greatly affects the interpretation of serological tests. Methods used in conventional viral purification and molecular cloning and expression of target viral proteins to obtain antigens for immunization are presented. Immunization of rabbits, mice and chickens and isolation of immunoglobulin from immunized animals also are described.

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http://dx.doi.org/10.1007/978-1-59745-062-1_4DOI Listing

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