A biological assay system for studying the nematicidal activity of chemicals has been devised using a model consisting of cucumber (Cucumis sativus L. cv. Long Marketer) seedlings growing in the diSPo(R) growth-pouch apparatus. Meloidogyne incognita was used as the test organism. The response was quantified in terms of the numbers of galls produced. Statistical procedures were applied to estimate the ED(50) values of currently available nematicides. This system permits accurate quantification of galling and requires much less space and effort than the currently used methods.
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Arch Pharm (Weinheim)
January 2025
European Institute for Molecular Imaging (EIMI), University of Muenster, Muenster, Germany.
The P2X4 receptor (P2X4R), a ligand-gated ion channel activated by ATP, plays a critical role in neuroinflammation, chronic pain, and cancer progression, making it a promising therapeutic target. In this study, we explored the design and synthesis of piperazine-based P2X4R antagonists, building on the structural framework of paroxetine. A series of over 35 compounds were synthesized to investigate structure-activity relationships (SARs) in a Ca²⁺-flux assay for P2X4R antagonistic activity.
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December 2024
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Plant-parasitic nematodes pose a significant threat to crop production, impacting agricultural yields. In the search for new nematicides, a series of 1,2,4-oxadiazole-5-carboxylic acid derivatives containing amide or ester groups were designed and synthesized using an activity-based approach. Bioassay results showed that some compounds exhibited good nematicidal activity against , , and .
View Article and Find Full Text PDFJ Basic Microbiol
December 2024
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Research on nematode management globally highlights the use of nematicidal biomolecules and biocontrol agents. However, the availability of biomolecules to manage plant-parasitic nematodes remains limited. The discovery of microbial biomolecules offers new opportunities in this field, though they are underexplored for suppressing nematodes.
View Article and Find Full Text PDFNat Commun
December 2024
Beijing Life Science Academy, Beijing, China.
Nipah virus (NiV) is a non-segmented, negative-strand (NNS) RNA virus, belonging to Paramyxoviridae. The RNA polymerase complex, composed of large (L) protein and tetrameric phosphoprotein (P), is responsible for genome transcription and replication by catalyzing NTP polymerization, mRNA capping and cap methylation. Here, we determine the cryo-electron microscopy (cryo-EM) structure of fully bioactive NiV L-P polymerase complex at a resolution of 3.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
December 2024
Institute BIOTECMED/Department of Genetics, University of Valencia, Burjassot, Spain.
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