AI Article Synopsis
- A high-throughput assay utilizing PCR and electrospray ionization-mass spectrometry (PCR/ESI-MS) was developed to genotype Staphylococcus aureus isolates.
- The primers were designed to amplify genes that are also analyzed in multilocus sequence typing (MLST), allowing for identification of clonal complexes and USA types.
- This method can process over 180 samples daily and has been validated against established techniques like MLST and PFGE, showing consistent results across diverse clinical isolates.
Article Abstract
We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (MLST). The method was used to identify the clonal complex and USA type of each isolate and is suitable for use in a clinical or public-health setting. The method was validated using a panel of diverse isolates from the Centers for Disease Control and Prevention that were previously characterized by MLST and pulsed-field gel electrophoresis (PFGE). Clinical isolates from two geographically distinct hospitals were characterized, and the clustering results were in agreement with those for repetitive-element PCR and PFGE. The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691079 | PMC |
| http://dx.doi.org/10.1128/JCM.02175-08 | DOI Listing |
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