AI Article Synopsis
- The study used constant current chronopotentiometry to analyze protein denaturation at a mercury electrode.
- In a 50 mM sodium phosphate solution at pH 7, proteins like bovine serum albumin remained stable and did not denature.
- However, at higher phosphate concentrations, the proteins experienced denaturation due to the influence of the electric field on the electrode surface.
Article Abstract
Using constant current chronopotentiometry we showed that in 50 mM sodium phosphate (pH 7) bovine serum albumin and some other proteins were not significantly denatured at a bare mercury electrode while at higher phosphate concentrations they underwent electric field-driven denaturation on the electrode surface.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/b822274f | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multi-Analytical Approach for the Acid-Base, Thermal and Surface Properties Assessment of Waste Biomasses.
Molecules
December 2024
Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche e Ambientali, Università degli Studi di Messina, 98168 Messina, Italy.
A multi-analytical approach was used to comprehensively characterize the acid-base, thermal, and surface properties of agri-food processing wastes (i.e., original and pre-treated bergamot, grape and olive pomaces).
View Article and Find Full Text PDFPathological Mutations D169G and P112H Electrostatically Aggravate the Amyloidogenicity of the Functional Domain of TDP-43.
ACS Chem Neurosci
December 2024
Physical and Materials Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
Article Synopsis
- TDP-43 aggregation is linked to neurodegenerative diseases like ALS, with specific mutations (D169G and P112H) affecting its stability and aggregation.
- The P112H mutant exhibits higher chemical stability at physiological pH but forms amyloid fibrils more rapidly at low pH compared to TDP-43 and D169G.
- This study combines experimental and simulation techniques to reveal how the mutations influence the conformational changes and aggregation process of TDP-43, highlighting the role of ionic strength and charge in the aggregation behavior.
Binding between Cu/Zn and aged polyethylene and polyethylene terephthalate microplastics in swine wastewaters: Adsorption behavior, and mechanism insights.
Environ Pollut
November 2024
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, 219 Ningliu Road, Nanjing 210044, China; NUIST Reading Academy, Nanjing University of Information Science and Technology, 219 Ningliu Road, Nanjing 210044, China. Electronic address:
Microplastics (MPs) have aroused growing environmental concerns due to their biotoxicity and vital roles in accelerating the spread of toxic elements. Illuminating the interactions between MPs and heavy metals (HMs) is crucial for understanding the transport and fate of HM-loaded MPs in specific environmentally relevant scenarios. Herein, the adsorption of copper (Cu) and zinc (Zn) ions over polyethylene (PE) and polyethylene terephthalate (PET) particulates before and after heat persulfate oxidation (HPO) treatment was comprehensively evaluated in simulated and real swine wastewaters.
View Article and Find Full Text PDFThe Impact of Polyphosphates on the Colloidal Stability of Laponite Particles.
J Phys Chem B
July 2024
MTA-SZTE Momentum Biocolloids Research Group, Department of Physical Chemistry and Materials Science, Interdisciplinary Centre of Excellence, University of Szeged, 6720 Szeged, Hungary.
The effect of polyphosphate (polyP) adsorption on the colloidal properties of disc-shaped laponite (LRD) particles was examined in aqueous dispersions with a focus on elucidating the interparticle forces that govern the colloidal stability of the systems. The charge and aggregation rate data of bare LRD exhibited an ionic strength-dependent trend, confirming the presence of double-layer repulsion and van der Waals attraction as major surface interactions. The charge of LRD particles significantly increased in magnitude at elevated polyP concentrations as a result of polyP adsorption and subsequent overcharging of the positively charged sites on the edges of the LRD discs.
View Article and Find Full Text PDFThe substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact.
Elife
May 2024
Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Bacteria utilize various strategies to prevent internal dehydration during hypertonic stress. A common approach to countering the effects of the stress is to import compatible solutes such as glycine betaine, leading to simultaneous passive water fluxes following the osmotic gradient. OpuA from is a type I ABC-importer that uses two substrate-binding domains (SBDs) to capture extracellular glycine betaine and deliver the substrate to the transmembrane domains for subsequent transport.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!