Electrophoretic fluorescence images have been fabricated by deposition of nanoporous polyaniline-fluorescein conducting particles onto a micro-patterned polyaniline film.
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Synthesis of zwitterionic asymmetric and symmetric carboxy-imidazolium derivatives and their use in molecular interactions with bovine serum albumin.
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A. M. Butlerov Institute of Chemistry, Kazan Federal University, Kremlevskaya str. 18, Kazan 420018, Russia.
For the first time asymmetric and symmetric carboxytriazoleimidazolium derivatives with different structures were synthesized. The critical micellization concentration (CMC) value was estimated using a pyrene fluorescent probe and the solubility of Orange OT. The complexation ability of carboxytriazoleimidazolium derivatives toward bovine serum albumin (BSA) has been investigated by various physico-chemical methods: fluorescence spectroscopy, electrophoretic light scattering and circular dichroism.
View Article and Find Full Text PDFEffect of the operational parameters on the electromigration of proteins in sodium dodecyl sulfate capillary gel electrophoresis in the presence of propidium iodide fluorescent dye.
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January 2025
Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary; Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary. Electronic address:
Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) is a frequently used analytical technique in size-based separation of proteins, playing a vital role in the biopharmaceutical industry for the analysis and characterization of therapeutic proteins, employing both UV and fluorescent detection. Understanding the effect of the operational parameters using easily applicable in migratio fluorescent labeling is increasingly critical, especially because multicapillary electrophoresis systems with fluorescent detection have recently gained prominence in high-throughput biopolymer analysis. In this study, the effects of the three most important user-adjustable operational parameters (temperature, gel concentration, and electric field strength) were investigated on the electrophoretic mobility and resolution of SDS-protein complexes in the presence of propidium iodide in the gel-buffer system.
View Article and Find Full Text PDFAGEing of collagen: The effects of glycation on collagen's stability, mechanics and assembly.
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Department of Physics, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada. Electronic address:
Advanced Glycation End Products (AGEs) are the end result of the irreversible, non-enzymatic glycation of proteins by reducing sugars. These chemical modifications accumulate with age and have been associated with various age-related and diabetic complications. AGEs predominantly accumulate on proteins with slow turnover rates, of which collagen is a prime example.
View Article and Find Full Text PDFEffect of daphnetin, the coumarin derivative isolated from Daphne genus, on Helicobacter pylori adhesion to gastric epithelial cells.
Phytomedicine
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Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100073, China. Electronic address:
Background: Adherence of Helicobacter pylori to the surface of the gastric mucosa is the initial and crucial step for its survival and colonization in the harsh conditions of the stomach. We had previously demonstrated that daphnetin has anti-adhesion effect.
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SlUPA-like, a bHLH Transcription Factor in Tomato (), Serves as the Crosstalk of GA, JA and BR.
Int J Mol Sci
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Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400044, China.
The bHLH (basic Helix-Loop-Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of in L. Ailsa Craig (AC) altered the JA (Jasmonic acid) response and endogenous GA (Gibberellic acid) content.
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