We examined the hypothesis that human mesenchymal stem cells detect physiological mechanical signals. Human bone marrow stromal cells (HBMSCs) were exposed to fluid shear stress of 12 dynes/cm(2) and analysed for their ability to express osteoblast-specific markers and associated signalling pathways. HBMSCs showed a significant increase in alkaline phosphatase (ALP) gene expression and a marked decrease in type I collagen, while no effect on Cbfa1/Runx2 was detected. This regulation is related to p38 and ERK1/2 activation, although the use of specific inhibitors to these two MAP kinases suggests that ALP mRNA induction is especially dependent on p38 activity, while type I collagen downregulation is ERK1/2-dependent. Interestingly, the expression of connexin43, which is involved in cell-to-cell communication of osteoblastic cells through gap junction formation, and its distribution through the cells, were modified by fluid flow (FF). HBMSCs are sensitive to shear stress and it appears essential to take their responsiveness into consideration before associating these regenerative cells with a bioactive biomaterial in a new bone tissue-engineering strategy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/term.166 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigating the Detachment of Glazed Ceramic Tiles Used in Buildings: A Brazilian Case Study.
Materials (Basel)
January 2025
School of Engineering, Mackenzie Presbyterian University, São Paulo 01302-907, Brazil.
Ceramic detachments in cladding systems are indicative of adhesion loss between the ceramic tiles and the substrate or its adhesive mortar due to inadequate quality workmanship, the quality of the adhesive mortar or that of the ceramic material, whether acting simultaneously or not. The shear stresses resulting from the ceramic tiles' expansion due to humidity accelerate this process. There is a shortage of studies on the quality of ceramic tiles and adhesive mortars.
View Article and Find Full Text PDFMacro-Micro Mechanics of Granular Soils Under Shear Considering Coupled Effects of Particle Size Distribution and Particle Morphology.
Materials (Basel)
January 2025
Department of Architecture and Civil Engineering, City University of Hong Kong, Hong Kong.
This paper investigates the effects of particle morphology (PM) and particle size distribution (PSD) on the micro-macro mechanical behaviours of granular soils through a novel X-ray micro-computed tomography (μCT)-based discrete element method (DEM) technique. This technique contains the grain-scale property extraction by the X-ray μCT, DEM parameter calibration by the one-to-one mapping technique, and the massive derivative DEM simulations. In total, 25 DEM samples were generated with a consideration of six PSDs and four PMs.
View Article and Find Full Text PDFDeformation Behavior of Inconel 625 Alloy with TPMS Structure.
Materials (Basel)
January 2025
School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200082, China.
Triply periodic minimal surfaces (TPMSs) are known for their smooth, fully interconnected, and naturally porous characteristics, offering a superior alternative to traditional porous structures. These structures often suffer from stress concentration and a lack of adjustability. Using laser powder bed fusion (LPBF), we have fabricated Inconel 625 sheet-based TPMS lattice structures with four distinct topologies: Primitive, IWP, Diamond, and Gyroid.
View Article and Find Full Text PDFStability and Rheological Properties of Grouts with Waste Glass Powder as Cement Replacement: Influences of Content and Alkali Activator.
Materials (Basel)
January 2025
College of Civil Engineering, Changsha University of Science and Technology, Changsha 410114, China.
Effective recycling and utilization of waste glass is a critical issue that urgently needs to be addressed. This study aims to explore the feasibility of using ground waste glass powder (particle size ≤ 75 μm) as a supplementary cementitious material to partially replace cement in the preparation of low-carbon and environmentally friendly grouting materials. The research systematically evaluates the impact of waste glass powder (WGP) on the fresh properties (particularly the stability and rheological characteristics) of cement-based grouting materials under various conditions, including WGP content (0-40%), the addition of NaOH activator (NaO content of 4%) or not, and water-solid ratio (/ 0.
View Article and Find Full Text PDFExpansion Characteristics and Shear Behavior of Reinforced Concrete Beams Under Non-Uniform Expansion Induced by Alkali-Silica Reaction.
Materials (Basel)
January 2025
Engineering Research Centre of Diagnosis Technology of Hydro-Construction, Chongqing Jiaotong University, Chongqing 400074, China.
Alkali-silica reaction (ASR) is an important factor that seriously affects the durability of reinforced concrete (RC) structures. The current research on alkali-aggregate mainly focuses on the deterioration mechanism of materials and the mechanical properties of standard specimens. However, there is a gap in the field of research on the effect of alkali-aggregate damage on the level of RC structures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!