AI Article Synopsis
- A study analyzed polymorphisms in the HSP70-1 gene of 253 Chinese Holstein dairy cows to explore their relationship with somatic cell scores (SCS), which indicate mastitis susceptibility.
- Researchers discovered two silent mutations (G-->A-->C at 1,623 bp and G-->A at 2,409 bp) that did not change the amino acid sequence and found that the 1,623 bp locus significantly influenced SCS.
- Results showed that cows with the CC genotype had lower SCS and were deemed mastitis resistant, suggesting that the CC genotype may serve as a genetic marker for improving anti-mastitis traits in this breed.
Article Abstract
The polymorphisms of HSP70-1 gene in 253 Chinese Holstein dairy cows were studied, and the association between the polymorphisms and somatic cell score (SCS) were analyzed. PCR-SSCP, PCR-RFLP and DNA sequencing were used to investigate mutations in the coding region of HSP70-1 gene. The G-->A-->C mutation at 1 623 bp and G-->A mutation at 2 409 bp were found and both of them were silence mutations that caused no alteration in amino acid sequence. Chi-square test showed both loci were'nt at Hardy-Weinberg disequilibrium in Chinese Holstein. In the meanwhile, the association of 2 409 locus and SCS was not significant. However, the polymorphism at 1623 locus affected SCS significantly (P<0.05). The SCS of genotype CC was significantly lower than that of genotype AG and GG (P<0.05), so genotype CC was mastitis resistant. These results suggest that genotype CC of HSP70-1 gene may be used as a molecular and genetic marker to improve the phenotype of anti-mastitis in Chinese Holstein dairy cows.
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| http://dx.doi.org/10.3724/sp.j.1005.2009.00169 | DOI Listing |
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