A practical, economical, and industrial process for the enzymatic production of difructose anhydride III (DFA III) was investigated for crude inulin prepared from chicory roots using Arthrobacter sp. H65-7 fructosyltransferase. A comparable level of DFA III production to that from commercial inulin was obtained using crude inulin, suggesting the feasibility of this production process.
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http://dx.doi.org/10.1016/j.jbiosc.2008.11.013 | DOI Listing |
Appl Microbiol Biotechnol
November 2024
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima, 890-0065, Japan.
Di-fructofuranose 1,2':2,3' dianhydride (DFA-III) is a cyclic fructo-disaccharide, which is produced by the condensation of two fructose molecules via the caramelization or enzymatic reaction of inulin fructotransferase. A strain of Blautia producta was known to utilize DFA-III as a carbohydrate source; however, the mechanisms remain unclear. In this study, we characterized the glycoside hydrolase (GH) family 91 DFA-III hydrolase (DFA-IIIase) from B.
View Article and Find Full Text PDFInt J Biol Macromol
November 2024
School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China. Electronic address:
The functional Difructose anhydride III (DFA-III) lacks reported utilization by special probiotics of Bifidobacterium and Lactobacillus. DFA-III hydrolase (DFA-IIIase), converting DFA-III to inulobiose, is a critical enzyme for the metabolism of DFA-III, which stands for the utilization ability of DFA-III by microorganisms. Hence, the research identified six potential DFA-IIIases from Bifidobacterium and Lactobacillus species, suggesting that DFA-III has the potential to proliferate these bacteria.
View Article and Find Full Text PDFJ Dairy Sci
July 2024
ICREA, 08007 Barcelona, Spain. Electronic address:
The objective of this study was to assess the potential effect of difructose anhydride III (DFAIII) on calcemia, magnesemia, and milking performance in dairy cows. A total of 66 multiparous Holstein cows in late pregnancy (gestation days, mean ± SD = 253.8 ± 2.
View Article and Find Full Text PDFAnimals (Basel)
December 2023
ICREA, Passeig de Lluís Companys 23, 08007 Barcelona, Spain.
The objective of this study was to assess the potential effects of supplementing difructose anyhdride III (DFAIII) during the first days of life on the absorption of immunoglobulin G (IgG) and growth performance of calves early in life fed colostrum with a high IgG concentration. Sixty-six healthy new-born Holstein calves were randomly assigned to three treatments consisting of no supplementation (control), supplementation of 12 g/d (DFA12), or 36 g/d (DFA36) of DFAIII during the first 7 d of life via colostrum and milk replacer (MR). Calves were separated from dams at birth and bottle-fed colostrum in two meals, each targeting 2.
View Article and Find Full Text PDFFood Chem
May 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, School of Internet of Things Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Inulin fructotransferase converts prebiotic polysaccharide inulin to difructose anhydride III, known for its numerous beneficial physiological effects. While previous studies focused on using inulin extracts under optimal conditions, this study delves into the enzyme's behavior when dealing with more complex food materials, inulin-rich burdock root, which possesses greater nutritional value but may influence the enzymatic reaction. An inulin fructotransferase from Arthrobacter sp.
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