Effect of Lactobacillus casei Shirota strain intraperitoneal administration in CD1 mice on the establishment of Trichinella spiralis adult worms and on IgA anti-T. spiralis production. | LitMetric
The effect of the intraperitoneal (ip) administration of Lactobacillus casei Shirota strain (LcS) in CD1 mice on the establishment of Trichinella spiralis adult worms (TSAW), and on the generation of intestinal IgA anti-T. spiralis after challenge (AC) were evaluated. One hundred and twenty mice were allocated at random into two groups of 60 mice each: Treated group (T) and Non-treated group (NT). Each mouse in T group was inoculated with LcS at days -21, -14, and -7. On day 0 each mouse was challenged with 200 larvae of T. spiralis. At days 3, 5, 7, 10, 12, 14, 17, 19, 21, and 28 AC, six mice from each group were sacrificed to obtain TSAW. At days 7, 14, 21, and 28 IgA-s anti-T. spiralis levels in intestinal washings were evaluated by ELISA. From day five on AC, mice in LcS group showed significantly less TSAW (P<0.05) than animals from NT group. At days 7, 14, 21, and 28 AC IgA anti-T. spiralis levels were higher in mice from T group (P<0.05) than in the NT group. The results indicate that LcS inoculated into mice induces protection against T. spiralis and an increase in the production of IgA anti-T. spiralis.
Human trichinosis is a serious foodborne parasitic zoonosis. Diagnosing human trichinosis is usually difficult due to the nonspecific clinical picture and the limited effectiveness of serological tests in acute infections. While ELISA can detect circulating Trichinella antigens, aiding in early diagnosis, its sensitivity may be low.
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address:
Trichinellosis is a serious foodborne and zoonotic parasitic disease caused by Trichinella family. At present, the main on-site detection method for Trichinella spiralis (T. spiralis) infection is the lateral flow assay (LFA).
[1,3,5]Triazino[1,2-]benzimidazole-2-amines bearing heterocyclic moiety in 4-position were synthesized. The compounds were characterized by elemental analysis, IR, H-NMR, C-NMR, and HRMS spectroscopy. The molecular geometry and electron structure of these molecules were theoretically studied using density functional theory (DFT) methods.
IgE antibodies are likely involved in host protection. is a helminth that induces protection through IgE antibodies. The present study examined susceptibility in high and low IgE responder mice, with a specific focus on the inheritance of IgE responsiveness, which controls IgE production specific for the IgE isotype and non-specific for antigens.
Human trichinellosis is a worldwide foodborne public health threat. Detecting circulating antigens of "" allows for an early diagnosis before larval encystation develops in skeletal muscles. For the first time, the present study aimed to formulate an effective nanomagnetic beads based-ELISA and -latex agglutination test (NMB-ELISA and NMB-LAT) to recognize adult worm crude extract antigen (AWCEA) in sera of experimentally infected mice.
