AI Article Synopsis
- Recombineering is a technique that uses the functions of gamma phage for DNA manipulation in E. coli.
- It simplifies the creation of knockout vectors by allowing targeted edits in any genomic area.
- The text introduces a detailed protocol for making mouse conditional knockout targeting vectors using recombineering methods.
Article Abstract
Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by gamma phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706932 | PMC |
| http://dx.doi.org/10.1007/978-1-59745-471-1_2 | DOI Listing |
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