[Effects of uremia serum on monolayer permeability and F-actin in rat pulmonary microvascular endothelial cells].

Objective: To observe the effects of uremia serum on monolayer permeability and F-actin in cultured rat pulmonary microvascular endothelial cell (RPMVEC) and to investigate the mechanism of injury of RPMVEC induced by uremia serum.

Methods: RPMVEC was isolated and cultured from Wistar rat in vitro. The effects of serum of uremia patients on monolayer permeability of RPMVEC were observed with microfilter, and F-actin expression was evaluated by flow cytometry.

Results: Serum of uremia patients induced the increased permeability of RPMVEC monolayer for 6 h and 12 h (P < 0.01), depolymerization of F-actin for 6 h and 12 h (P < 0.01), the increased permeability of RPMVEC monolayer induced by serum of uremia patients is significantly correlated with the depolymerization of F-actin (r = -0.927, P < 0.01).

Conclusion: Serum of uremia patients may induce the elevation of permeability monolayer and the depolymerization of F-actin in RPMVEC. The elevation of permeability monolayer induced by serum of uremia patients in RPMVEC is significantly correlated with the depolymerization of F-actin.