Objective: To study the influence of circadian Clock gene on the fertilization ability of sperm via attenuating the expression of Clock with RNAi in male mice.
Methods: After the injection of RNAi plasmid into mice testes, the expression of circadian Clock gene of testis tissue was determined by western Blotting. The fertilization ability of sperm was evaluated by various indices, including litter size in mated female mice, sperm count, sperm motility, in vitro fertilization (IVF) rate and acrosome development.
Results: The RNAi plasmid targeting circadian gene Clock attenuated the expression of CLOCK in mice testis, reduced in vitro fertilization CIVF) rate and the conception rate in viva, and also decreased sperm acrosin activity.
Conclusion: Circadian gene Clock is related to the reproductive function in male mice. It probably affect sperm fertilization ability by regulating sperm acrosin activity.
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