AI Article Synopsis
- Selected reaction monitoring (SRM) MS is becoming a key method for targeted quantitative proteomics.
- Utilizing proteotypic peptides in SRM analysis reduces redundancy at both the sequence and mass-to-charge levels.
- This article emphasizes the significant challenges of designing effective SRM assays while addressing the issue of potential ion redundancy.
Article Abstract
Selected reaction monitoring (SRM) MS is proving to be a popular approach for targeted quantitative proteomics. The use of proteotypic peptides as candidates for SRM analysis is a wise first step in SRM method design. The obvious reason for this is the need to avoid redundancy at the sequence level, however this is incidental. The true reason is that homologous peptides result in redundancy in the mass-to-charge domain. This may seem like a trivial subtlety, however, we believe this is an issue of far greater significance than the proteomic community is aware. This VIEWPOINT article serves to highlight the complexity associated with designing SRM assays in light of potential ion redundancy.
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| http://dx.doi.org/10.1002/pmic.200800577 | DOI Listing |
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