AI Article Synopsis
- The ThPOD1 gene from Tamarix hispida is linked to stress responses like cold, salt, and drought, suggesting a role in dealing with environmental challenges.
- Expression of ThPOD1 was shown to be triggered by abiotic stresses and the hormone abscisic acid (ABA).
- Experiments with engineered plasmids found that ThPOD3 enhances E. coli’s resistance to drought, indicating potential applications for improving drought tolerance in plants through genetic modification.
Article Abstract
The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS-PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were approximately 57, approximately 50 and approximately 47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
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| http://dx.doi.org/10.1007/s11033-009-9484-8 | DOI Listing |
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