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Spherical harmonics texture extraction for versatile analysis of biological objects.

PLoS Comput Biol

January 2025

European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, Germany.

The characterization of phenotypes in cells or organisms from microscopy data largely depends on differences in the spatial distribution of image intensity. Multiple methods exist for quantifying the intensity distribution - or image texture - across objects in natural images. However, many of these texture extraction methods do not directly adapt to 3D microscopy data.

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Hyperspectral images (HSI) have been extensively applied in a multitude of domains, due to their combined spatial and spectral characteristics along with a wealth of spectral bands. The ingenious combination of spatial and spectral information in HSI classification has remained a central research area for an extended period. In the classification process, it is essential to choose an expanded neighborhood window for learning.

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Internet of Things (IoT) is one of the most important emerging technologies that supports Metaverse integrating process, by enabling smooth data transfer among physical and virtual domains. Integrating sensor devices, wearables, and smart gadgets into Metaverse environment enables IoT to deepen interactions and enhance immersion, both crucial for a completely integrated, data-driven Metaverse. Nevertheless, because IoT devices are often built with minimal hardware and are connected to the Internet, they are highly susceptible to different types of cyberattacks, presenting a significant security problem for maintaining a secure infrastructure.

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Deep learning is a double-edged sword. The powerful feature learning ability of deep models can effectively improve classification accuracy. Still, when the training samples for each class are limited, it will not only face the problem of overfitting but also significantly affect the classification result.

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Spectral Imaging techniques such as Laser-induced Breakdown Spectroscopy (LIBS) and Raman Spectroscopy (RS) enable the localized acquisition of spectral data, providing insights into the presence, quantity, and spatial distribution of chemical elements or molecules within a sample. This significantly expands the accessible information compared to conventional imaging approaches such as machine vision. However, despite its potential, spectral imaging also faces specific challenges depending on the limitations of the spectroscopy technique used, such as signal saturation, matrix interferences, fluorescence, or background emission.

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