AI Article Synopsis
- A study tested polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) on a panel of 156 bacterial isolates, mainly pathogens.
- The technique accurately identified 91% of the isolates at the genus level and 74% at the species level, with only 9% misidentified.
- This research indicates that multilocus PCR/ESI-MS could be a valuable method for identifying various bacterial types effectively.
Article Abstract
Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2008.12.012 | DOI Listing |
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