Mouse embryonic stem (ES) cells derived from pluripotent early epiblast contribute functionally differentiated progeny to all foetal lineages of chimaeras. By contrast, epistem cell (EpiSC) lines from post-implantation epithelialised epiblast are unable to colonise the embryo even though they express the core pluripotency genes Oct4, Sox2 and Nanog. We examined interconversion between these two cell types. ES cells can readily become EpiSCs in response to growth factor cues. By contrast, EpiSCs do not change into ES cells. We exploited PiggyBac transposition to introduce a single reprogramming factor, Klf4, into EpiSCs. No effect was apparent in EpiSC culture conditions, but in ground state ES cell conditions a fraction of cells formed undifferentiated colonies. These EpiSC-derived induced pluripotent stem (Epi-iPS) cells activated expression of ES cell-specific transcripts including endogenous Klf4, and downregulated markers of lineage specification. X chromosome silencing in female cells, a feature of the EpiSC state, was erased in Epi-iPS cells. They produced high-contribution chimaeras that yielded germline transmission. These properties were maintained after Cre-mediated deletion of the Klf4 transgene, formally demonstrating complete and stable reprogramming of developmental phenotype. Thus, re-expression of Klf4 in an appropriate environment can regenerate the naïve ground state from EpiSCs. Reprogramming is dependent on suppression of extrinsic growth factor stimuli and proceeds to completion in less than 1% of cells. This substantiates the argument that EpiSCs are developmentally, epigenetically and functionally differentiated from ES cells. However, because a single transgene is the minimum requirement to attain the ground state, EpiSCs offer an attractive opportunity for screening for unknown components of the reprogramming process.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2685927 | PMC |
http://dx.doi.org/10.1242/dev.030957 | DOI Listing |
J Environ Manage
December 2024
School of Business Administration, Chongqing Vocational College of Light Industry, Chongqing, 400065, China. Electronic address:
Green technology innovation (GTI) breaks the vicious cycle of "economic development-environmental pollution," mitigating the supply chain carbon emissions. Previous research focused on exploring supply chain GTI decision-making in the discrete strategy space and ignored the effect of stochastic factors. This paper, grounded in the classical evolutionary game theory, explores the interaction mechanism of supply chain GTI decision-making between suppliers and manufacturers under stochastic interferences and in the continuous strategy space.
View Article and Find Full Text PDFProbl Radiac Med Radiobiol
December 2024
State Institution «National Research Center for Radiation Medicine, Hematology and Oncology of the National Academy of Medical Sciences of Ukraine», 53 Yuriya Illienka Street, Kyiv, 04050, Ukraine.
Parathyroids are the key regulators of calcium-phosphorus metabolism. By means of parathyroid hormone they respond to any changes in the serum level of calcium and phosphorus ions and determine the integrity of skeleton, affecting almost all systems and cells where calcium and phosphorus are involved in metabolism and/or signaling.Disorders of parathyroid function are associated with significant complications accompanying secondary hyperparathyroidism.
View Article and Find Full Text PDFOtolaryngol Head Neck Surg
December 2024
Department of Otolaryngology-Head and Neck Surgery University of Pennsylvania Health System, Philadelphia, Pennsylvania, USA.
Objective: Clinicians face challenges in managing the growing population of patients with limited English proficiency (LEP) and hearing loss (HL) in the United States. This study seeks to investigate provider perspectives on evaluating, counseling, and treating HL in LEP patients.
Study Design: Prospective descriptive study.
PLoS One
December 2024
Department of Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America.
Vocalizations are one of the key premating reproductive barriers that could affect species formation. In song-learning birds, vocal traits are sometimes overlooked in species delimitation, as compared to morphological or plumage-based differences. In this study, we assessed geographic variation in songs of eight pairs of oscines on two scales: (1) comparing primary songs of species/subspecies pairs whose breeding grounds are eastern and western counterparts of each other in the continental North America, and (2) for each counterpart, identifying and comparing possible variation among their populations.
View Article and Find Full Text PDFFront Psychol
December 2024
Department of Psychological Science, Kennesaw State University, Kennesaw, GA, United States.
With the rapid expansion of mindfulness and its incorporation into the "third wave" of Cognitive Behavioral Therapy (CBT), there has been evident confusion about what mindfulness is and how it relates to this broader category of interventions. In this article, I define mindfulness and CBT, and differentiate them while highlighting their substantial overlap. Specifically, I discuss the Buddhist Psychological Model and how it relates to the foundational cognitive model, demonstrating the common threads that run across these seemingly disparate philosophies.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!