AI Article Synopsis
- Supported lipid bilayers (SLBs) are useful for studying membrane proteins and their functions, particularly in high-throughput screening for drug interactions with transmembrane proteins.
- The formation of SLBs involves rupturing anionic vesicles in the presence of Ca(2+) ions on ITO-coated surfaces, which allows for the characterization of their properties.
- The platform developed enables controlled assembly and regeneration of SLBs on ITO microelectrode arrays, facilitating the study of membrane processes using electric potential switching.
Article Abstract
Supported lipid bilayers (SLBs) are ideal platforms for the study of membrane proteins and function. Assembly of functional SLBs in an array format would lead to a breakthrough in high-throughput screening of membrane-associated processes, e.g., drugs binding to transmembrane proteins. We report the formation of SLBs from the rupture of anionic vesicles in the presence of Ca(2+) ions on ITO-coated surfaces and characterise the assembly and SLB properties. Furthermore, the formation, manipulation and regeneration of SLBs adsorbed on ITO microelectrode array spots using an electric potential switch are demonstrated. This platform enables addressable assembly and the study of electrochemically mediated membrane processes in a microarray format which can be regenerated in situ.
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| http://dx.doi.org/10.1039/b814281e | DOI Listing |
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