The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.
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http://dx.doi.org/10.1007/s00436-009-1358-4 | DOI Listing |
Parasit Vectors
December 2024
College of Public Health, University of Iowa, Iowa City, IA, USA.
Background: Visceral leishmaniosis (VL) is the most severe form of human leishmaniosis, with an estimated 95% case fatality if left untreated. Dogs act as peridomestic reservoir hosts for the protozoan parasite Leishmania infantum, a causative agent for human leishmaniosis, endemic throughout the Mediterranean basin. To assure consistent and accurate surveillance of canine infection and prevent transmission to people, consistent diagnosis of canine L.
View Article and Find Full Text PDFBMC Vet Res
December 2024
Departamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Escola de Medicina Veterinária e Zootecnia, Av. Milton Santos 500, Salvador, Bahia, CEP 40170-110, Brazil.
Background: Ehrlichia spp. are obligate intracytoplasmic Gram-negative tickborne bacteria from the Anaplasmataceae family. Ehrlichiosis is considered an emerging disease in humans and animals.
View Article and Find Full Text PDFVet Parasitol
December 2024
CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal; Elvas School of Biosciences, Polytechnic Institute of Portalegre, Portugal; VALORIZA - Research Centre for Endogenous Resources Valorisation, Polytechnic Institute of Portalegre, Portugal.
Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Theileria equi, Theileria haneyi, and Babesia caballi. EP is endemic in most tropical and subtropical regions worldwide, and there is a likelihood that it is also endemic in Portugal. This retrospective study aimed to determine the seroprevalence, prevalence, and potential risk factors of EP in our country over the past five years.
View Article and Find Full Text PDFPLoS One
December 2024
Faculty of Veterinary Medicine, Department of Veterinary Sciences, Institute for Infectious Diseases and Zoonoses, Chair of Bacteriology and Mycology, Ludwig-Maximilians-Universität Munich, Oberschleißheim, Bavaria, Germany.
Diagnosis of equine Lyme borreliosis (LB), an infection caused by members of the Borrelia burgdorferi sensu lato complex (Bbsl), is challenging due to the nonspecific clinical signs of the disease and due to the variety of non-standardized serological tests. Specific vaccine-induced antibodies against LB, providing an effective protection against the infection, complicate the issue further. The standard for the detection of specific antibodies against Bbsl is a two-tier test system based on an enzyme-linked immunosorbent assay (ELISA) or indirect fluorescent antibody test (IFA) for antibody screening combined with a qualitative, highly specific immunoassay (e.
View Article and Find Full Text PDFbioRxiv
December 2024
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, United States.
In cardiac muscle, many myosin molecules are in a resting or "OFF" state with their catalytic heads in a folded structure known as the interacting heads motif (IHM). Many mutations in the human β-cardiac myosin gene that cause hypertrophic cardiomyopathy (HCM) are thought to destabilize (decrease the population of) the IHM state. The effects of pathogenic mutations on the IHM structural state are often studied using indirect assays, including a single-ATP turnover assay that detects the super-relaxed (SRX) biochemical state of myosin functionally.
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