AI Article Synopsis
- RNA interference is a way that cells can control their genes, using tiny molecules called microRNA.
- The study found that a specific microRNA made by HIV-1 helps infected cells survive longer by stopping them from dying, which allows the virus to reproduce more.
- This TAR microRNA is found at all stages of the HIV life cycle and is present even in cells that are not actively making the virus.
Article Abstract
Background: RNA interference is a gene regulatory mechanism that employs small RNA molecules such as microRNA. Previous work has shown that HIV-1 produces TAR viral microRNA. Here we describe the effects of the HIV-1 TAR derived microRNA on cellular gene expression.
Results: Using a variation of standard techniques we have cloned and sequenced both the 5' and 3' arms of the TAR miRNA. We show that expression of the TAR microRNA protects infected cells from apoptosis and acts by down-regulating cellular genes involved in apoptosis. Specifically, the microRNA down-regulates ERCC1 and IER3, protecting the cell from apoptosis. Comparison to our cloned sequence reveals possible target sites for the TAR miRNA as well.
Conclusion: The TAR microRNA is expressed in all stages of the viral life cycle, can be detected in latently infected cells, and represents a mechanism wherein the virus extends the life of the infected cell for the purpose of increasing viral replication.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654423 | PMC |
| http://dx.doi.org/10.1186/1742-4690-6-18 | DOI Listing |
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