AI Article Synopsis
- * The soybeans that were developed showed resistance to the herbicide bispyribac-sodium, confirming that they expressed the Os-mALS gene properly through genetic testing.
- * Despite some unintended soybean plants without the resistant gene appearing, the overall system using the herbicide to select for transgenic soybeans proved effective for production.
Article Abstract
We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T(1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene.
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| http://dx.doi.org/10.1007/s00299-009-0679-1 | DOI Listing |
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