A densely packed gold nanoparticle platform combined with a multiple-enzyme labeled detection antibody-magnetic bead bioconjugate was used as the basis for an ultrasensitive electrochemical immunosensor to detect cancer biomarkers in serum. Sensitivity was greatly amplified by synthesizing magnetic bioconjugates particles containing 7500 horseradish peroxidase (HRP) labels along with detection antibodies (Ab2) attached to activated carboxyl groups on 1 microm diameter magnetic beads. These sensors had sensitivity of 31.5 microA mL ng(-1) and detection limit (DL) of 0.5 pg mL(-1) for prostate specific antigen (PSA) in 10 microL of undiluted serum. This represents an ultralow mass DL of 5 fg PSA, 8-fold better than a previously reported carbon nanotube (CNT) forest immunosensor featuring multiple labels on carbon nanotubes, and near or below the normal serum levels of most cancer biomarkers. Measurements of PSA in cell lysates and human serum of cancer patients gave excellent correlations with standard ELISA assays. These easily fabricated AuNP immunosensors show excellent promise for future fabrication of bioelectronic arrays.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2666939 | PMC |
| http://dx.doi.org/10.1021/nn800863w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
2D wrinkle assisted zigzag plasmonic chains for isotropic SERS enhancement.
Sci Rep
January 2025
Institute of Physical Chemistry and Polymer Physics, Leibniz-Institut für Polymerforschung Dresden e.V. (IPF), 01069, Dresden, Germany.
Template-assisted colloidal self-assembly has gained significant attention due to its flexibility and versatility. By precisely controlling the shape of the template, it is possible to achieve custom-designed nanoparticle assemblies. However, a major challenge remains in fabricating these templates over large areas at a low cost.
View Article and Find Full Text PDFThree-dimensional diffractive acoustic tomography.
Nat Commun
January 2025
Department of Biomedical Engineering, Duke University, Durham, NC, USA.
Acoustically probing biological tissues with light or sound, photoacoustic and ultrasound imaging can provide anatomical, functional, and/or molecular information at depths far beyond the optical diffusion limit. However, most photoacoustic and ultrasound imaging systems rely on linear-array transducers with elevational focusing and are limited to two-dimensional imaging with anisotropic resolutions. Here, we present three-dimensional diffractive acoustic tomography (3D-DAT), which uses an off-the-shelf linear-array transducer with single-slit acoustic diffraction.
View Article and Find Full Text PDFA miniaturized colorimetric-photoacoustic dual-mode device for point-of-care testing of ascorbic acid and ascorbate oxidase.
Anal Chim Acta
March 2025
The Radiology Department of Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, China. Electronic address:
Background: Real-time and rapid detection of ingredients in food has important significance for food safety. However, traditional detection methods not only require bulky and costly instruments but also are often based on single-mode analysis, limiting their accuracy and applications in point-of-care testing. Herein, an integrated and miniaturized dual-mode device based on colorimetric and photoacoustic (PA) principles is developed, using Au@Ag nanoparticles (Au@AgNPs) as signal probe and ascorbic acid (AA) and ascorbate oxidase (AAO) as analytes.
View Article and Find Full Text PDFA machine-learning-integrated portable electrochemiluminescence sensing platform for the visualization and high-throughput immunoassays.
Talanta
January 2025
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, PR China. Electronic address:
Electrochemiluminescence (ECL)-based point-of-care testing (POCT) has the potential to facilitate the rapid identification of diseases, offering advantages such as high sensitivity, strong selectivity, and minimal background interference. However, as the throughput of these devices increases, the issues of increased energy consumption and cross-contamination of samples remain. In this study, a high-throughput ECL biosensor platform with the assistance of machine learning algorithms is developed by combining a microcolumn array electrode, a microelectrochemical workstation, and a smartphone with custom software.
View Article and Find Full Text PDFCutting-edge: bionanomaterial solutions in the battle against Severe Acute Respiratory Syndrome Coronavirus 2.
Braz J Biol
January 2025
Near East University, Operational Research Center in Healthcare, Mersin, Turkey.
Amidst the ongoing COVID-19 pandemic, the imperative of our time resides in crafting stratagems of utmost precision to confront the relentless SARS-CoV-2 and quell its inexorable proliferation. A paradigm-shifting weapon in this battle lies in the realm of nanoparticles, where the amalgamation of cutting-edge nanochemistry begets a cornucopia of inventive techniques and methodologies designed to thwart the advances of this pernicious pathogen. Nanochemistry, an artful fusion of chemistry and nanoscience, provides a fertile landscape for researchers to craft innovative shields against infection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!