[Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC].

Objective: To develog an HPLC method determination of two new saponins in the dried rhizomes of Tupistra chinensis.

Method: The analysis was performed on YMC-Pack ODS-AQ (4.6 mm x 250 mm, 5 microm) eluted with of acetonitrile and waterin gradient mode. The conentration of acetonitrile in the mobile phase changes from 10% to 100% within 85 minutes. The detection wavelength was set at 203 nm. The flow rate was 1.00 mL x min(-1) and column temperature was set at 30 degrees C.

Result: The linear relationships of two authentic saponins were determined within the range from 7.55 microg to 60.40 microg (r=0.9996) and 8.00 microg to 48.00 microg (r=0.9996), respectively. Using the method above, the contents of two saponins were determined as 0.261% and 0.242%, with the recoveries as 98.6% and 98.3% with RSD 1.7% and 1.7%, respectively.

Conclusion: A convenient and reliable method was developed to determine the content of two saponins in the dried rhizomes of Tupistra chinensis.