Study of Costus lucanusianus: plant juice, fraction combinations and pharmacologic estimation of natural product total activity.

The juice of Costus lucanusianus fresh stems was split by chromatography using pharmacological measurements on duodenum and uterus to follow activity. The concentration-effect curves of the parent juice and the active fraction alone or associated with other fractions were analysed according to classical methods used for combination drugs. Results show that the active fraction is potentialised by two groups of fractions which are without intrinsic activity.