Objective: To investigate the change in renal high mobility group box-1 protein (HMGB1) levels, and the effect of Chinese traditional medicine-Xuebijing injection on HMGB1 expression as well as acute kidney injury in rats after scald injury.
Methods: Wistar rats were subjected to 30% full-thickness scald injury followed with delayed resuscitation. Totally 78 animals were divided into sham scald group (n=18), scald injury group (n=30), and Xuebijing injection treatment group (n=30). All animals were sacrificed at 8, 24, and 72 hours postburn. Renal tissue and blood samples were harvested to determine HMGB1 mRNA as well as protein expression and organ functional parameters. HMGB1 mRNA level was semi-quantitatively measured by the reverse transcription polymerase chain reaction taking GAPDH as an internal standard, and protein expressions of HMGB1 were detected by both Western blot and immunohistochemistry. Serum creatinine (Cr) contents were measured by automatic biochemistry analyzer. In addition, pathological lesions in kidney were observed under light microscope using HE staining.
Results: Compared with sham scald group, both mRNA and protein expressions of HMGB1 were significantly enhanced in the kidney at 8, 24, and 72 hours after scald injury (P<0.05, P<0.01), meanwhile serum Cr contents were markedly increased following acute insults (P<0.05, P<0.01). Treatment with Xuebijing injection could markedly down-regulated renal HMGB1 mRNA expression and protein release at 24 hours and 72 hours (P<0.05, P<0.01), and significantly reduced serum Cr content following scald injury (P<0.05). Many inflammatory cells in renal tissues were observed using light microscope following scald. The histological morphology of kidney lesions was a-HMGB1, a late mediator, appears to be inmeliorated after treatment with Xuebijing injection.
Conclusions: volved in the pathogenesis of excessive inflammatory response and acute kidney damage. Treatment with Xuebijing injection can inhibit HMGB1 synthesis and release in renal tissues, and may prevent the development of acute kidney injury induced by serious scald injury.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The total extract of Abelmoschus manihot (L.) medic flowers (TEA) mediated Nrf2-TFAM signalling to regulate mitochondrial antioxidant mechanism.
Sci Rep
January 2025
Department of Pharmacology, Zhejiang University of Technology, 18 Chao-Wang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
Skin, as the first line of defence of the human body, is exposed to dangers such as overheating substances, ultraviolet rays, and environmental pollutants, and the incidence of skin diseases is increasing annually. Oxidative stress plays a dominant role in most skin diseases. Abelmoschus manihot (L.
View Article and Find Full Text PDFTrends and epidemiology of children treated in specialized burn centers in the Netherlands between 2009 and 2022.
Eur J Pediatr
January 2025
Alliance of Dutch Burn Care, Burn Center, Red Cross Hospital, PO Box 1074, 1940 EB, Beverwijk, the Netherlands.
Article Synopsis
- The study examines trends and epidemiology of pediatric burns in Dutch burn centers from 2009 to 2022, highlighting the need for effective prevention strategies and resource allocation.
- The analysis revealed a gradual increase in pediatric burn admissions, with a temporary decrease during the COVID-19 pandemic; 77% of patients were overnight admissions.
- Young children (ages 0-3) were the most affected group, and a shift toward shorter hospital stays and increased day admissions was noted, especially during the pandemic.
Fiber optic laryngoscopy findings in pediatric burn patients referred to the pediatric emergency department.
Am J Emerg Med
December 2024
Department of Pediatric Emergency Medicine Department, Hadassah Medical Center, Jerusalem, Israel.
Introduction: Facial burn injuries can compromise the airways in pediatric patients. Because prompt assessment of airway safety is a must, most assessment algorithms rely mainly on clinical judgment. There is little data on the value or utility of Fiber Optic Laryngoscopy (FOL) as an ancillary test.
View Article and Find Full Text PDFXeroform gauze versus silver sulfadiazine for mixed-depth pediatric scald injuries: A retrospective study.
JPRAS Open
March 2025
Department of Surgery, Division of Pediatric Surgery, Children's Hospital of Michigan/Wayne State University School of Medicine, Detroit, MI, USA.
Introduction: Silver sulfadiazine 1 % cream had historically been the mainstay initial treatment for scald wounds at our institution. However, we transitioned to using closed dressings of only petrolatum-impregnated 3 % bismuth tribromophenate gauze (Xeroform) for all partial-thickness burns. Xeroform adheres to the wound while allowing the exudates to drain and acts as a scaffold for re-epithelialization, after which it falls off without traumatizing the wound bed, theoretically allowing viable tissue to declare itself while requiring less frequent dressing changes.
View Article and Find Full Text PDFMassage Therapy Utilization in Pediatric Acute Burns: A Retrospective Cohort Study.
Int J Ther Massage Bodywork
December 2024
Division of Pediatric Surgery, The Burn Center, Nationwide Children's Hospital, Columbus, OH, USA.
Background: Patient-centered burn care extends beyond physical treatment to incorporate the management of the psychological impacts including stress, pain, and anxiety. This study explores the novel application of massage therapy (MT) in children with acute burns, assessing utilization and impact on pain and relaxation.
Methods: A retrospective review of 198 children with thermal injury admitted to an American Burn Association-verified pediatric burn center between January 2022 and July 2023 was conducted, excluding those requiring intensive care admission.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!