AI Article Synopsis
- An improved analytical assay for milrinone quantification was developed for patients undergoing cardiac surgery, using solid-phase extraction and HPLC with UV detection.
- Plasma samples were processed with a C(18) cartridge and separated on a strong cation exchange column, achieving consistent calibration curves within the range of 1.25-320 ng/ml.
- The method demonstrated high drug recovery (≥96%) and accuracy (≥92%), with low intra- and inter-day precision (≤6.7% and ≤7.9%), making it reliable and specific for milrinone measurements, especially for inhalation administration.
Article Abstract
An improved analytical assay was developed and validated for the quantification of milrinone concentrations in patients undergoing cardiac surgery. A solid-phase extraction was optimized to isolate milrinone from a plasma matrix followed by HPLC using UV detection. Plasma samples (1 ml) were extracted using a C(18) solid-phase cartridge. Milrinone was separated on a strong cation exchange analytical column maintained at 23.4 degrees C. The mobile phase consisted of a gradient (10:90 to 45:55), 0.05 M phosphate buffer (pH 3):acetonitrile. Calibration curves were linear in the concentration range of 1.25-320 ng/ml. Mean drug recovery and accuracy were respectively > or =96% and > or =92%. Intra- and inter-day precisions (CV%) were < or =6.7% and < or =7.9%, respectively. This method proved to be reliable, specific and accurate. Using different types of column for extraction and separation of milrinone proved to be necessary to achieve the sensitivity and specificity required when milrinone is given by inhalation.
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| http://dx.doi.org/10.1016/j.jchromb.2009.01.024 | DOI Listing |
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