Two new scorpion species, Ananteris faguasi sp. nov. and Ananteris volschenki sp. nov., are described from the eastern extreme of El Tuparro Natural National Park, eastern Colombia near the border with Venezuela. These new species are the first of the genus to be known from Vichada department, and represent the easternmost records in the country. With their description, the number of known species of Ananteris is raised to 64, ten of which are present in Colombia. A map with the known distribution of the Colombian species of Ananteris is presented.
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