AI Article Synopsis
- - The study involved creating two new strains of Saccharomyces cerevisiae that combined the SDH1 gene's regulatory region with the E. coli lacZ gene, which produces beta-galactosidase.
- - Researchers targeted a specific yeast strain, flx1 delta-lacZ, that does not have a working mitochondrial FAD translocator known as Flx1p, to investigate SDH1 gene expression.
- - The findings support the idea that there is a link between imbalances in flavin cofactors and the expression of mitochondrial apo-flavoproteins.
Article Abstract
We described here the construction of two novel Saccharomyces cerevisiae strains in which the regulatory region of the SDH1 gene, coding for the succinate dehydrogenase flavoprotein subunit, was fused in frame to the reporter gene lacZ of E. coli, coding for beta-galactosidase. By this approach, SDH1 expression was studied in the yeast strain, flx1 delta-lacZ, lacking of a functional mitochondrial FAD translocator, Flx1p. The experiments described here are in line with the hypotesys that a correlation exists between defects in flavin cofactor homeostasis and mitochondrial apo-flavoprotein expression.
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