AI Article Synopsis
- 1-Bromopropane (1-BP) is used as a replacement for harmful chlorofluorocarbons, but its neurotoxic effects are not well understood.
- The study found that 1-BP significantly lowered brain-derived neurotrophic factor (BDNF) levels in astrocytes from both human and mouse sources.
- It was observed that 1-BP reduces the activity of essential molecules like CREB and protein kinase A (PKA), which play roles in regulating BDNF expression.
Article Abstract
1-Bromopropane (1-BP) has been widely used as an alternative to ozone-depleting chlorofluorocarbons in various industries. Although the neurotoxicity of 1-BP has been recently reported, there is little information about the effect of 1-BP on the cells in brain by experimental approach. Here we studied the effect of 1-BP on brain-derived neurotrophic factor (BDNF) expression in astrocytes in vitro. The BDNF mRNA level was remarkably decreased by 1-BP in a human astrocytoma cell line, U251, and in mouse primary astrocytes. The DNA-binding and specific reporter activity of cAMP response element-binding transcription factor (CREB), which is one of the key molecules regulating BDNF expression, were reduced by 1-BP in U251 and/or mouse primary astrocytes. Additionally, protein kinase A (PKA) activity was suppressed by 1-BP in U251. These results suggest that BDNF expression was affected by 1-BP through at least PKA.
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| http://dx.doi.org/10.1016/j.intimp.2009.01.007 | DOI Listing |
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