AI Article Synopsis
- Flat aberrant crypt foci (flat ACF) and mucin-depleted foci (MDF) are early signs of potential cancer in rodent colons, identified using specific staining methods.
- A study examined the locations of these lesions in Min mice and F344 rats treated with cancer-causing agents, comparing the results from two different staining techniques.
- The findings revealed a significant overlap between the two types of lesions, with 57% in the mice and 42% in the rats, indicating that while they frequently coincide, they are not always the same.
Article Abstract
Background: Flat aberrant crypt foci (flat ACF) and mucin-depleted foci (MDF) are preneoplastic lesions identified in the colon of carcinogen-treated rodents stained with methylene blue (MB) and high iron diamine-alcian blue (HID-AB), respectively. The correspondence between flat ACF and MDF in the same colon of Min mice treated with azoxymethane (AOM) and of F344 rats treated with 1,2-dimethylhydrazine (DMH) was explored.
Materials And Methods: The position of each flat ACF was recorded on a digitally constructed photographic map of the MB-stained colon. The same colons were then stained with HID-AB and the position of each MDF was compared with that of flat ACF.
Results: The fraction of coincident lesions, identified as both flat ACF and MDF with the two staining methods, was 57% and 42%, in the Min mice and F344 rats, respectively. Flat ACF or MDF not coincident with the two staining methods were either undetectable or ACF with one of the two methods.
Conclusion: Flat ACF and MDF show considerable, but not total, overlap.
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