As a widely used and expensive herbal medicine, Panax ginseng has many adulterants in the commercial market. PCR-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) based on 5S rDNA sequence analysis were applied to identify two common adulterants of P. ginseng. The sizes of 5S rRNA gene non-transcribed spacers (NTS) sequences in P. ginseng and its adulterants were determined, ranging from 143 to 424 bp. The PCR product of P. ginseng only could be digested among the tested specimens because of its specific SpeI restriction site found in the 5S rDNA sequence. In addition, P. ginseng was successfully identified from compound medicinal preparations and from the Single-Taste medicines. These results suggest that the methods are able to authenticate P. ginseng.
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Article Synopsis
- Panax notoginseng powder (PNP) is a valuable medicinal product, but its effectiveness is compromised by widespread adulteration in the market.
- This study aims to create a quality evaluation system using artificial intelligence sensory and multi-source information fusion technologies to accurately assess different grades of PNP and its adulterated varieties.
- The results showed a 100% accuracy in identifying PNP grades and adulteration, with highly precise predictions for adulteration ratios and total saponin content, indicating a reliable method for evaluating PNP quality.
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