The aim of the present study is to examine the effects of the anti-tumor component isolated from Trichosanthes kirilowii on human hepatocellular carcinoma cells. Using Sephadex G-25 column chromatography, Sep-Pak Plus C18 cartridge and high-performance liquid chromatography (HPLC), we isolated the active component from trichosanthes extract. By fast atom bombardment mass spectrometric analysis, the molecular mass of the active fraction was determined, the active components identified, and their mechanisms of action were analyzed by cell growth assay, cell cycle analysis, TUNEL staining and Western blot analysis. We found that the anti-tumor components isolated from the extract of trichosanthes (EOT) are cucurbitacin D and dihydrocucurbitacin D, and suggest that cucurbitacin D induces apoptosis through caspase-3 and phosphorylation of JNK in hepatocellular carcinoma cells. These results suggest that cucurbitacin D isolated from Trichosanthes kirilowii could be a valuable candidate for anti-tumor drug.
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http://dx.doi.org/10.1016/j.intimp.2009.01.006 | DOI Listing |
Arch Virol
October 2024
Department of Horticulture, Foshan University, Foshan, Guangdong, 528225, P. R. China.
Plant Dis
July 2024
University of North Bengal, Department of Botany, Darjeeling, West Bengal, India;
Plant Dis
June 2024
Anhui Academy of Agricultural Sciences, Plant Protection and Agro-Products Safety, Hefei, Anhui, China.
Maxim. (), one of the Chinese herbal medicines, is an economically important crop in Anhui Province, China. In recent years, gummy stem blight disease, a major disease of cucurbits, was widespread in many plantations.
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August 2024
National Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Ministry of Education Key Laboratory of Pharmacology of Traditional Chinese Medicine Formulae, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:
Plant Dis
February 2024
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing, Beijing, China;
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