Agricultural biotechnology companies have been asked to provide intact transgenic seed to regulatory agencies as reference materials for evaluating transgene and protein detection methods (PCR and immunoassay). Due to intellectual-property and product-stewardship considerations, submission of devitalized seed prior to regulatory approval is preferable in any given country. Commonly used devitalization procedures, such as heating or autoclaving, degrade the protein and/or DNA rendering the seed unfit as a reference material for these tests. A novel method for devitalizing seed was developed that involves hydration, freezing in liquid nitrogen, and lyophilization. The devitalization method described here was found to preserve the transgenic DNA and protein in cotton (Gossypium hirsutum) and maize (Zea mays) seed allowing its use as a reference material for evaluating detection methods.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628069 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton.
Physiol Plant
January 2025
Zhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Mitogen-activated protein kinase kinase kinase kinases (MAP4Ks) are a class of highly conserved serine/threonine-protein kinases in eukaryotes. They participate in the typical MAPK cascade system and various signal transduction pathways regulating biological processes in plants, during stressful conditions. To date, genome-wide identification of MAP4Ks in cotton has not been reported.
View Article and Find Full Text PDFOverexpression of apple MdNRT1.7 enhances low nitrogen tolerance via the regulation of ROS scavenging.
Int J Biol Macromol
December 2024
Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, Guizhou Province, China; College of Agriculture, Guizhou Engineering Research Center for Fruit Crops, Guizhou University, Guiyang 550025, Guizhou Province, China. Electronic address:
Low nitrogen stress significantly limits crop production. The role of NRT1.7 as a nitrate transporter in alleviating low nitrogen stress in apple (Malus domestica) remains unclear.
View Article and Find Full Text PDFPomegranate ATP-binding cassette transporter PgABCG9 plays a negative regulatory role in lignin accumulation.
Int J Biol Macromol
December 2024
Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-Construction by Ministry and Province), Key Laboratory of Horticultural Crop Genetic Improvement and Eco-physiology of Anhui Province, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China. Electronic address:
Seed hardness is an important quality characteristic of pomegranate fruit. The development of seed hardness relies on the deposition of lignin in the inner seed coat, but the underlying molecular mechanisms remain unclear. In this study, we identified a member of ABCG transporters, PgABCG9, which may function in seed hardening by negatively regulating lignin biosynthesis.
View Article and Find Full Text PDFAmino acid transporter GmAAP6-like contributes to seed quality and responds to jasmonic acid pathway under MSX toxicity stress.
Plant Physiol Biochem
December 2024
College of Plant Science, Jilin University, Changchun, 130062, China. Electronic address:
Amino acid transporters participate in the transport and distribution of amino acids in plants and are vital for plant growth and development. Despite their importance, few works have investigated the functions of amino acid permeases (AAP) amino acid transporters in soybean. In this study, we re-identified the AAP family genes in soybean using a new public genome database and cloned a soybean AAP gene renamed GmAAP6-like.
View Article and Find Full Text PDFOverexpression of NAC transcription factors from Eremopyrum triticeum promoted abiotic stress tolerance.
Transgenic Res
December 2024
College of Life Science, Shihezi University, Shihezi, Xinjiang, China.
Eremopyrum triticeum is a typical spring ephemeral species, which in China mainly distributed in the desert regions of northern Xinjiang, and play an important role in the desert ecosystems. E. triticeum has several adaptive characteristics such as short growth rhythms, high photosynthetic efficiency, high seed production, drought and salt resistance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!