Enterovirus 71 (EV71) can cause brain encephalitis and mortality. However, effective vaccines or chemotherapeutic agents are not yet available. We tested the hypothesis that Pueraria lobata could inhibit the cytotoxic effect of EV71 in a human foreskin fibroblast cell line by the XTT method. Our results showed that the water extract of P. lobata could inhibit cytopathy induced by EV71 when given before (p < 0.0001), simultaneously with (p < 0.0001), or after viral infection (p < 0.0001). Water extract of P. lobata was effective and its minimal concentration that inhibited 50% of the cytopathic effect (IC50) was 0.028 microg/mL. P. lobata was also safe with a selectivity index greater than 107,000. Water extract of P. lobata appeared to inhibit viral attachment (p < 0.0001) and penetration (p < 0.0001). The anti-EV71 activity of the water extract of P. lobata was not mediated by interferons. In conclusion, the water extract of P. lobata was effective in the management of the disease induced by EV71 infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/S1607-551X(09)70011-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biofabrication of zinc oxide nanoparticles using Moringa oleifera, characterization and statistical optimization for their application in crystal violet adsorption.
Sci Rep
January 2025
Department of Biology, College of Sciences and Arts Khulais, University of Jeddah, 21959, Jeddah, Saudi Arabia.
Crystal violet (Cry) is an essential textile dye belonging to the triphenylmethane group, that is widely used in the textile industry. It is also applied for paper printing and Gram staining. Previously, it was significant as a topical antiseptic due to its antibacterial, antifungal, and anthelmintic properties.
View Article and Find Full Text PDFIdentifying falsified COVID-19 vaccines by analysing vaccine vial label and excipient profiles using MALDI-ToF mass spectrometry.
NPJ Vaccines
January 2025
Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, UK.
The rapid development and worldwide distribution of COVID-19 vaccines is a remarkable achievement of biomedical research and logistical implementation. However, these developments are associated with the risk of a surge of substandard and falsified (SF) vaccines, as illustrated by the 184 incidents with SF and diverted COVID-19 vaccines which have been reported during the pandemic in 48 countries, with a paucity of methods for their detection in supply chains. In this context, matrix-assisted laser desorption ionisation-time of flight (MALDI-ToF) mass spectrometry (MS) is globally available for fast and accurate analysis of bacteria in patient samples, offering a potentially accessible solution to identify SF vaccines.
View Article and Find Full Text PDFMicrobial indicators for water quality in recirculating shower technology.
Sci Total Environ
January 2025
Technical University of Denmark, DTU Sustain, Bygningstorvet, Building 115, 2800 Kg. Lyngby T. Pérez Guillemette, Denmark. Electronic address:
Recirculating showers save up to 70-80 % of the water and energy use of conventional showers, but water quality in these systems are not studied very well due to the technology's early stages. The aim of this study was to provide an overview of information available on which microorganisms were investigated and at which densities. Based on this platform we further aimed at identifying key microbial indicators and pathogens for monitoring water quality in these systems by integrating data from the top five waterborne outbreaks with findings from studies on (1) microbial investigations in reuse projects involving shower effluents or combined bathroom streams, (2) shower water effluents, and (3) biofilms in conventional showers.
View Article and Find Full Text PDFA moisture differential technique for extraction and maintenance of imported fire ant colonies under laboratory conditions.
Sci Rep
January 2025
National Center for Natural Products Research, The University of Mississippi, Mississippi, 38677, MS, USA.
Establishing and maintaining colonies of imported fire ants (IFA) (Hymenoptera: Formicidae) in the laboratory are crucial for research. Dehydration is one of the major mortality factors in IFA, and the ants tend to relocate from dry to moist places. In our laboratory, we developed a moisture differential technique to extract fire ant colonies from mound materials.
View Article and Find Full Text PDFIsolation of Natural Products from Marine Invertebrates.
Methods Mol Biol
January 2025
Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, Busan, Republic of Korea.
Marine invertebrates such as sponges, cnidarians, bryozoans, and tunicates are considered to be prolific sources of novel biologically active natural products. Specimens from these organisms are composed of soft tissues with a high water and inorganic salt content compared to those from terrestrial counterparts. In addition, some of the secondary metabolites from these organisms tend to be more polar than those of terrestrial.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!