[Identification of porcine parvovirus and virus-specific antibodies by polymerase chain reaction and enzyme immunoassay].

Porcine parvovirus infection (PPVI) caused by a small non-enveloped virus (porcine parvovirus, PPV) is responsible for serious reproductive dysfunctions in sows. In the Russian Federation, the approved diagnostic methods for determination of parvovirus antigen and virus-specific antibodies are hemagglutination and hemagglutination-inhibition tests, respectively. The present paper gives the results of developing diagnostic tests to reveal porcine parvovirus by polymerase chain reaction and its antibodies by recombinant protein-based indirect enzyme immunoassay. The developed tests supplement a battery of diagnostic methods and specify some disputable results obtained by classical methods.