AI Article Synopsis
- Bacillus subtilis mutants were isolated that showed resistance to peptide deformylase inhibitors due to mutations in three specific genes necessary for formylation of Met-tRNA(fMet).
- These mutations in the fmt, folD, and glyA genes allowed the bacteria to bypass the formylation process, resulting in significant fitness losses, although the overall transcription profile remained unchanged.
- Despite the reduced fitness, there were minor adaptations observed in the enzymes of intermediary metabolism within the mutant strains.
Article Abstract
Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNA(fMet), fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was observed.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663100 | PMC |
| http://dx.doi.org/10.1128/AAC.01340-08 | DOI Listing |
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