The present study was carried out to examine global gene expression in the brainstem, in a mouse facial carrageenan injection model of orofacial pain. Mice that received facial carrageenan injection showed increased mechanical allodynia, demonstrated by increased responses to von Frey hair stimulation of the face. The brainstem was harvested at 3 days post-injection, corresponding to the time of peak responses, and analyzed by Affymetrix Mouse Genome 430 2.0 microarrays. We sought to identify common genes that are changed in the respective sides of the brainstem after either right- or left-sided facial carrageenan injection. The result is a relatively small list of genes (22 genes), which were then classified using DAVID software. Many of them fell into the categories of "response to stress", "defence response", "response to biotic stimulus", "cell adhesion" and "leukocyte adhesion". Of these, increased expression of P-selectin, ICAM-1 and CCL12 after carrageenan injection could be verified by real-time RT-PCR on both the right and left sides, and increased in P-selectin and ICAM-1 further verified by Western blot analysis. P-selectin and ICAM-1 were immunolocalized to endothelial cells, and were double labelled with von Willebrand factor. Intraperitoneal injection of the P-selectin inhibitor KF38789 significantly reduced mechanical allodynia in the facial carrageenan-injected mice. P-selectin mediates the capturing of leukocytes from the bloodstream and rolling of leukocytes along the endothelial surface. We hypothesize that increased nociceptive input to the brainstem could attract circulating macrophages into the brain, resulting in neuroinflammation and pain.
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http://dx.doi.org/10.1016/j.pain.2008.12.029 | DOI Listing |
Pain
August 2024
UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Facial grimacing is used to quantify spontaneous pain in mice and other mammals, but scoring relies on humans with different levels of proficiency. Here, we developed a cloud-based software platform called PainFace ( http://painface.net ) that uses machine learning to detect 4 facial action units of the mouse grimace scale (orbitals, nose, ears, whiskers) and score facial grimaces of black-coated C57BL/6 male and female mice on a 0 to 8 scale.
View Article and Find Full Text PDFACS Appl Bio Mater
June 2024
NanoBioTech Laboratory, Department of Environmental Engineering, Florida Polytechnic University, Lakeland, Florida 33805, United States.
Silver nanoparticles (AgNPs) are a potent antibacterial agent, especially when used to treat bacteria that are multidrug resistant. However, it is challenging to eliminate the hazardous reducing agents that remain in AgNPs produced by the conventional chemical reduction process. To overcome these challenges, the presented research demonstrates the fabrication of AgNPs using iota-carrageenan (ι-carra) as a carbohydrate polymer using electron beam (EB) irradiation.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
May 2024
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok 690022, Russia.
Polymer mucoadhesive films being developed for use in ophthalmology represent a new tool for drug delivery and are considered an alternative to traditional dosage forms. Due to their mucoadhesive properties, carrageenans (CRGs) are widely used in various forms for drug delivery. In this study, films based on CRGs of various structural types (κ/β, κ, x, and λ) for use in ophthalmology were studied.
View Article and Find Full Text PDFSensors (Basel)
November 2023
Department of Mechatronics, Robotics, and Digital Manufacturing, Vilnius Gediminas Technical University, 10105 Vilnius, Lithuania.
The development of low-cost biodegradable pressure or force sensors based on a carrageenan and iron (III) oxide mix is a promising way to foster the spread of green technologies in sensing applications. The proposed materials are inexpensive and abundant and are available in large quantities in nature. This paper presents the development and experimental study of carrageenan and iron (III)-oxide-based piezoresistive sensor prototypes and provides their main characteristics.
View Article and Find Full Text PDFFuture Microbiol
December 2023
Department of Medicine, University of Otago, 23A Mein Street, Wellington, 6021, New Zealand.
To observe upper respiratory tract infection (URTI) symptoms, rhinovirus levels and compliance with daily carrageenan nasal spray. 102 adults were randomized to carrageenan or saline placebo three times daily for 8 weeks and URTI symptoms were recorded. A control group (n = 42) only recorded URTI symptoms.
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