AI Article Synopsis
- The study compared the efficiencies of three derivatisation reagents—FMOC, butanol, and propyl chloroformate—in analyzing amino acids using LC-ESI-MS/MS.
- The research focused on the separation of 20 amino acids and evaluated the method's linearity, repeatability, and detection limits for various derivatised forms.
- Propyl chloroformate showed the best performance, achieving lower detection limits and optimal linearity, making it the preferred reagent for analyzing amino acids in rat brain microdialysis samples.
Article Abstract
The efficiencies of three derivatisation reagents that react with either the amine (9-fluorenylmethyl chloroformate (FMOC)) or the carboxylic acid group (butanol) of amino acid or with both types of functional groups (propyl chloroformate) were compared in the analysis of amino acids by liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS). Separation of 20 amino acids derivatised with these three reagents was studied on reversed-phase chromatography. Linearity, repeatability and limits of detection of the LC-ESI-MS/MS method were determined by analysing FMOC-, butanol- and propyl chloroformate-derivatised lysine, beta-aminobutyric acid, threonine and glutamic acid. The limits of detection for the derivatised amino acids (7.5-75fmol) were as much as 2-60 times lower than those of the corresponding underivatised molecules. The best linearity was observed for amino acids derivatised with propyl chloroformate or butanol (r(2)=0.996-0.999, range=100-8500nmolL(-1)). Propyl chloroformate was the best suited of the reagents tested for the analysis of amino acids with LC-MS/MS and was used for the analysis of amino acids in rat brain microdialysis samples.
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| http://dx.doi.org/10.1016/j.aca.2008.11.055 | DOI Listing |
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