Objective: To establish HPLC fingerprints of cordyceps and mycelium of cultured cordy for quality control.
Method: The analysis was carried out on a Diamonsil C18 column eluted with phosphate buffer (pH 6.5) and methanol as mobile phase, the gradient elution program was used, and the detection wavelength was 260 nm; The similarity of fingerprints was evaluated, then, cluster analysis was studied.
Result: The method of HPLC fingerprint analysis was validated and in keeping with the requirement. Ten common peaks were found in HPLC fingerprint. Cordyceps from different areas and mycelium of cultured cordy could be distinguished by the HPLC fingerprint.
Conclusion: All results above exhibit that this method is practicable, reproducible, and reliable as a method for the quality control of cordyceps and mycelium of cultured cordy at present.
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